Microscopy image segmentation tool: Robust image data analysis

Abstract

We present a software package called Microscopy Image Segmentation Tool (MIST). MIST is designed for analysis of microscopy images which contain large collections of small regions of interest (ROIs). Originally developed for analysis of porous anodic alumina scanning electron images, MIST capabilities have been expanded to allow use in a large variety of problems including analysis of biological tissue, inorganic and organic film grain structure, as well as nano- and meso-scopic structures. MIST provides a robust segmentation algorithm for the ROIs, includes many useful analysis capabilities, and is highly flexible allowing incorporation of specialized user developed analysis. We describe the unique advantages MIST has over existing analysis software. In addition, we present a number of diverse applications to scanning electron microscopy, atomic force microscopy, magnetic force microscopy, scanning tunneling microscopy, and fluorescent confocal laser scanning microscopy.

Document Details

Document Type
Pub Defense Publication
Publication Date
Mar 01, 2014
Source ID
10.1063/1.4866687

Entities

People

  • Carlos Monton
  • Ilya Valmianski
  • Ivan K. Schuller

Organizations

  • Air Force Office of Scientific Research
  • University of California, San Diego

Tags

Fields of Study

  • Physics

Readers

  • Combustion and Flow Dynamics.
  • Computer Vision.
  • Thin Film Deposition Science.

Technology Areas

  • Directed Energy
  • Microelectronics