Solid-immersion fluorescence microscopy with increased emission and super resolution
Abstract
We investigate solid-immersion fluorescence microscopy suitable for super-resolution nanotechnology and biological imaging, and have observed limit of resolution as small as 15 nm with microspheres, mitochondria, and chromatin fibers. We have further observed that fluorescence efficiency increases with excitation power density, implicating appreciable stimulated emission and increased resolution. We discuss potential advantages of the solid-immersion microscopy, including combined use with previously established super-resolution techniques for reaching deeper beyond the conventional diffraction limit.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 05, 2015
- Source ID
- 10.1063/1.4905284
Entities
People
- A. A. Liau
- J. J. Chen
- J. M. Porter
- S. S. Sheu
- W. C. Salmon
- Z. L. Liau
Organizations
- Massachusetts Institute of Technology
- Thomas Jefferson University
- United States Department of Defense