A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices
Abstract
There is a growing interest to develop microfluidic bioreactors and organ-on-chip platforms with integrated sensors to monitor their physicochemical properties and to maintain a well-controlled microenvironment for cultured organoids. Conventional sensing devices cannot be easily integrated with microfluidic organ-on-chip systems with low-volume bioreactors for continual monitoring. This paper reports on the development of a multi-analyte optical sensing module for dynamic measurements of pH and dissolved oxygen levels in the culture medium. The sensing system was constructed using low-cost electro-optics including light-emitting diodes and silicon photodiodes. The sensing module includes an optically transparent window for measuring light intensity, and the module could be connected directly to a perfusion bioreactor without any specific modifications to the microfluidic device design. A compact, user-friendly, and low-cost electronic interface was developed to control the optical transducer and signal acquisition from photodiodes. The platform enabled convenient integration of the optical sensing module with a microfluidic bioreactor. Human dermal fibroblasts were cultivated in the bioreactor, and the values of pH and dissolved oxygen levels in the flowing culture medium were measured continuously for up to 3 days. Our integrated microfluidic system provides a new analytical platform with ease of fabrication and operation, which can be adapted for applications in various microfluidic cell culture and organ-on-chip devices.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 01, 2016
- Source ID
- 10.1063/1.4955155
Entities
People
- Adel Pourmand
- Akhtar Nadhman
- Ali Khademhosseini
- Amir Sanati Nezhad
- Eleanor Laukaitis
- Fabio De Ferrari
- John P. Ryan
- Mahboubeh Nabavinia
- Mehmet Remzi Dökmeci
- Niema Binth Mohammad
- Ramin Banan Sadeghian
- Seyed Ali Mousavi Shaegh
- Su Ryon Shin
- Yu Shrike Zhang
Organizations
- Defense Threat Reduction Agency
- Harvard Medical School
- Harvard University
- International Islamic University Islamabad
- King Abdulaziz University
- Konkuk University
- Massachusetts Institute of Technology
- National Heart, Lung, and Blood Institute
- National Institute of Allergy and Infectious Diseases
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of Biomedical Imaging and Bioengineering
- National Institute of Dental and Craniofacial Research
- Polytechnic University of Turin
- Tabriz University of Technology
- Tohoku University
- University of Calgary
- University of Toronto
- Wyss Institute for Biologically Inspired Engineering