Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures
Abstract
Integrating a perfusable microvasculature system in vitro is a substantial challenge for “on-chip” tissue models. We have developed an inclusive on-chip platform that is capable of maintaining laminar flow through porous biosynthetic microvessels. The biomimetic microfluidic device is able to deliver and generate a steady perfusion of media containing small-molecule nutrients, drugs, and gases in three-dimensional cell cultures, while replicating flow-induced mechanical stimuli. Here, we characterize the diffusion of small molecules from the perfusate, across the microvessel wall, and into the matrix of a 3D cell culture.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 01, 2016
- Source ID
- 10.1063/1.4963145
Entities
People
- Andre A. Adams
- Frances Ligler
- Kyle A. Divito
- Michael A. Daniele
- Steven A Roberts
Organizations
- Defense Threat Reduction Agency
- North Carolina State University
- Office of Naval Research
- United States Naval Research Laboratory