Squeezed light induced two-photon absorption fluorescence of fluorescein biomarkers
Abstract
Two-photon absorption (TPA) fluorescence of biomarkers has been decisive in advancing the fields of biosensing and deep-tissue in vivo imaging of live specimens. However, due to the extremely small TPA cross section and the quadratic dependence on the input photon flux, extremely high peak-intensity pulsed lasers are imperative, which can result in significant photo- and thermal damage. Previous works on entangled TPA with spontaneous parametric downconversion light sources found a linear dependence on the input photon-pair flux, but are limited by low optical powers, along with a very broad spectrum. We report that by using a high-flux squeezed light source for TPA, a fluorescence enhancement of ∼47 is achieved in fluorescein biomarkers as compared to classical TPA. Moreover, a polynomial behavior of the TPA rate is observed in the the laser dye 4-dicyanomethylene-2-methyl-6-(p(dimethylamino)styryl)-4H-pyran in dimethyl sulphoxide.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 22, 2020
- Source ID
- 10.1063/5.0010909
Entities
People
- Anton Classen
- Charles Altuzarra
- G. S. Agarwal
- Lin Fu
- Tian Li
Organizations
- Air Force Office of Scientific Research
- Texas A&M University
- University of Glasgow