High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity
Abstract
The brain is an especially active metabolic system, requiring a large supply of energy following neuronal activation. However, direct observation of cellular metabolic dynamics associated with neuronal activation is challenging with currently available imaging tools. In this study, an optical imaging approach combining imaging of calcium transients and the metabolic co-enzyme nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) is utilized to track the metabolic dynamics in hippocampal neuron cultures. Results show distinct cellular components for the NAD(P)H response following neuronal activity, where notable differences in the NAD(P)H dynamics between neurons and astrocytes can be directly observed. Additionally, tracking of these responses across a large field of view is demonstrated for metabolic profiling of neuronal activation. Observation of neuronal dynamics using these methods allows for closer examination of the complex metabolic machinery of the brain, and may lead to a better understanding of the cellular metabolism of neuronal activation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 22, 2021
- Source ID
- 10.1063/5.0031348
Entities
People
- Andrew J Bower
- Carlos Renteria
- Joanne Li
- Marina Marjanovic
- Ronit Barkalifa
- Stephen A. Boppart
Organizations
- Air Force Office of Scientific Research
- National Institutes of Health
- National Science Foundation
- University of Illinois Urbana–Champaign