Assessment of ABT-263 activity across a cancer cell line collection leads to a potent combination therapy for small-cell lung cancer

Abstract

Small-cell lung cancer (SCLC) is an aggressive carcinoma with few effective treatment options beyond first-line chemotherapy. BH3 mimetics, such as ABT-263, promote apoptosis in SCLC cell lines, but early phase clinical trials demonstrated no significant clinical benefit. Here, we examine the sensitivity of a large panel of cancer cell lines, including SCLC, to ABT-263 and find that high Bcl2-interacting mediator of cell death (BIM) and low myeloid cell leukemia 1 (MCL-1) expression together predict sensitivity. SCLC cells relatively resistant to ABT-263 are sensitized by TORC1/2 inhibition via MCL-1 reduction. Combination of ABT-263 and TORC1/2 inhibition stabilizes or shrinks tumors in xenograft models, in autochthonous SCLC tumors in a genetically engineered mouse model, and in a patient-derived xenograft SCLC model. Collectively, these data support a compelling new therapeutic strategy for treating SCLC.

Document Details

Document Type
Pub Defense Publication
Publication Date
Mar 03, 2015
Source ID
10.1073/pnas.1411848112

Entities

People

  • Aaron N Hata
  • Ah Ting Tam
  • Alan T. Yeo
  • Anahita Dastur
  • Anna F. Farago
  • Anthony C. Faber
  • Bethany L. Wagner
  • Brad E. Windle
  • Carlotta Costa
  • Charles T. Jakubik
  • Cyril H. Benes
  • Elena J. Edelman
  • Hiromichi Ebi
  • Hisashi Harada
  • Jeffrey A. Engelman
  • Jessica L. Boisvert
  • Jungoh Ham
  • Maria Gomez-caraballo
  • Mark A. Hicks
  • Miguel N. Rivera
  • Neha U. Patel
  • Randy J. Milano
  • Rebecca Robbins
  • Ryan J. March
  • Sarah Elmiligy
  • Scott E. Malstrom
  • Sridhar Ramaswamy
  • Tyler Jacks
  • William M. Rideout Iii
  • Youngchul Song

Organizations

  • American Cancer Society
  • Burroughs Wellcome Fund
  • Harvard Medical School
  • Lung Cancer Research Foundation
  • Massachusetts General Hospital
  • Massachusetts Institute of Technology
  • National Cancer Institute
  • United States Department of Defense
  • Uniting Against Lung Cancer
  • Virginia Commonwealth University

Tags

Fields of Study

  • Biology

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech