Reducing the genetic code induces massive rearrangement of the proteome
Abstract
Expanding the genetic code is an important aim of synthetic biology, but some organisms developed naturally expanded genetic codes over the course of evolution. To understand the selective advantage of genetically encoding more than 20 amino acids, we investigated the proteome-wide response to reducing the genetic code of Methanosarcina acetivorans from 21 to 20 amino acids. The data show how a natural proteome adapts to genetic code reduction and indicate that the selective value of an expanded genetic code is related to carbon source range and metabolic efficiency.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 17, 2014
- Source ID
- 10.1073/pnas.1420193111
Entities
People
- Dieter Söll
- Ilka U. Heinemann
- Jesse Rinehart
- Johannes G. Schäfer
- Katharina Riedel
- Laure Prat
- Martin Kucklick
- Patrick O’donoghue
Organizations
- Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada
- National Institute of General Medical Sciences
- TU Braunschweig
- University of Greifswald
- Western University
- Yale University