Quantum dot/antibody conjugates for in vivo cytometric imaging in mice
Abstract
One of the key questions in biology is understanding how cells move, interact, and evolve in living organisms. Tremendous efforts have been made to answer these questions in vitro, which have yielded a molecular-level understanding of cellular events. However, an increasing number of studies indicate that cellular activities need to be understood in the context of their natural environments. Single-cell labeling methods in use currently involve immunohistochemistry, genetic manipulation, or irradiation of mice, none of which reflect the native microenvironments. Here, we report quantum dot immunoconstructs that can be used for intravital imaging of single cells in unmanipulated mice and multiplexed in vivo cytometric analysis of rare cell populations.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 20, 2015
- Source ID
- 10.1073/pnas.1421632111
Entities
People
- Dai Fukumura
- Dan G Duda
- Elisabeth Niemeyer
- Hee-Sun Han
- Jayeeta Bhaumik
- Jian Cui
- John D Martin
- Margaret R. Martin
- Moungi Bawendi
- Rakesh Jain
- Sylvie Roberge
- Walid S. Kamoun
- Yuhui Huang
- Yunching Chen
Organizations
- American Cancer Society
- Harvard Medical School
- Massachusetts Institute of Technology
- National Cancer Institute
- National Science Foundation
- United States Army Research Laboratory
- United States Department of Defense