Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes
Abstract
The advance of biotechnology opens up greater possibilities of bioterror and bioerror. Here, we propose multiplexed safeguard switches rooted in the development of foundational genomic, regulatory, and metabolic technologies. Safeguard switches can be regulated by submicromolar small molecule(s) and combined in a modular fashion. The resulting safeguard strains show high fitness and low reversion rates. Moreover, two distinct classes of safeguard switches are orthogonal, providing a potential fail-safe mechanism. The safeguard technologies provide a practical and generic approach to containing engineered microbes within defined laboratory and/or industrial environments, and can in principle be used in the field as well.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 26, 2015
- Source ID
- 10.1073/pnas.1424704112
Entities
People
- Alba Ubide
- Giovanni Stracquadanio
- Haiping Hao
- Jef Boeke
- Joel S. Bader
- Katrina Caravelli
- Neta Agmon
- Woo Jin Choi
- Yizhi Cai
Organizations
- Johns Hopkins University
- New York University
- University of Edinburgh