Oxidative cyclizations in orthosomycin biosynthesis expand the known chemistry of an oxygenase superfamily
Abstract
Bacterial resistance to clinically relevant antibiotics has renewed public interest in identifying therapeutics with new scaffolds for the treatment of such infections. Analogs of orthosomycins could provide one such scaffold. One route to modifying these scaffolds is through rational engineering of the biosynthetic enzymes, requiring characterization of the biosynthetic pathway. A key feature of orthosomycin antibiotics is the orthoester linkage between carbohydrate groups, and our data suggest that a family of oxygenases is likely responsible for orthoester formation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 03, 2015
- Source ID
- 10.1073/pnas.1500964112
Entities
People
- Brian O. Bachmann
- Bryan L. Gitschlag
- Emilianne K. Mccranie
- Jarrod A. Smith
- Jeannette L. Mathieu
- Kathryn M McCulloch
- Maruf Sarwar
- T. M. Iverson
- Yu Du
Organizations
- American Heart Association
- Foundation for the National Institutes of Health
- Office of Naval Research
- Vanderbilt University