Laser-based three-dimensional multiscale micropatterning of biocompatible hydrogels for customized tissue engineering scaffolds
Abstract
In this paper we present results on 3D, multiscale laser machining of soft, transparent biomaterials suited for cellular growth and/or implantation. We use an ultrafast laser to generate high-resolution, 3D structures within the bulk of a transparent soft-biomaterial formulation that can support cell growth and allow cells to penetrate deep within the material. The structure is created by multiphoton absorption which, thanks to the clarity of the silk gels, is possible nearly 1 cm below the surface of the material. This depth represents an ∼10× improvement over other materials. The ability to create micrometer-scale voids over such a large volume has promising applications in the biomedical field and its efficacy was demonstrated both in vitro and in vivo.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 15, 2015
- Source ID
- 10.1073/pnas.1509405112
Entities
People
- Benedetto Marelli
- Benjamin P. Partlow
- David L. Kaplan
- Fiorenzo G Omenetto
- Jeannine Coburn
- Jessica P. Mondia
- Jodie E. Moreau
- Matthew B. Applegate
Organizations
- Office of Naval Research Global
- Tufts University