SutA is a bacterial transcription factor expressed during slow growth in Pseudomonas aeruginosa
Abstract
Pathogens that are dormant or growing slowly play important roles in chronic infections, but studying how cells adapt to these conditions is difficult experimentally. This work demonstrates that time-selective analysis of cellular protein synthesis, using bioorthogonal noncanonical amino acid tagging (BONCAT), can provide the sensitivity needed to identify important factors in slow-growth physiology. We identified in Pseudomonas aeruginosa , a previously uncharacterized transcriptional regulator that is expressed preferentially under slow-growth conditions, binds RNA polymerase, and has widespread effects on gene expression. This factor is one of several proteins of unknown function identified in our proteomic analysis, and our results suggest that further characterization of fundamental cellular processes under these conditions will shed light on important and understudied realms of biology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 19, 2016
- Source ID
- 10.1073/pnas.1514412113
Entities
People
- Annie Moradian
- Brett M Babin
- David A. Tirrell
- Dianne Newman
- Megan Bergkessel
- Michael J. Sweredoski
- Sonja Hess
Organizations
- Army Research Office
- California Institute of Technology
- Gordon and Betty Moore Foundation
- National Institutes of Health