Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires
Abstract
We applied a very recently developed experimental strategy for high-throughput sequencing of paired antibody heavy and light chains along with large-scale computational structural modeling to delineate features of the human antibody repertoire at unprecedented scale. Comparison of antibody repertoires encoded by peripheral naive and memory B cells revealed ( i ) preferential enrichment or depletion of specific germline gene combinations for heavy- and light-chain variable regions and ( ii ) enhanced positive charges, higher solvent-accessible surface area, and greater hydrophobicity at antigen-binding regions of mature antibodies. The data presented in this report provide fundamental new insights regarding the biological features of antibody selection and maturation and establish a benchmark for future studies of antibody responses to disease or to vaccination.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 25, 2016
- Source ID
- 10.1073/pnas.1525510113
Entities
People
- Andrew D Ellington
- Brandon J DeKosky
- Constantine Chrysostomou
- Daechan Park
- Daisuke Kuroda
- Erik L. Johnson
- George Georgiou
- Gregory C Ippolito
- Jeffrey J. Gray
- Oana I. Lungu
- Wissam Charab
Organizations
- Defense Threat Reduction Agency
- Hertz Foundation
- Johns Hopkins University
- National Institutes of Health
- National Science Foundation
- University of Texas at Austin