Human neural crest cells contribute to coat pigmentation in interspecies chimeras after in utero injection into mouse embryos
Abstract
We generated mouse–human neural crest chimeras by introducing neural crest cells derived from human embryonic stem cells or induced pluripotent stem cells (iPSCs) in utero into the gastrulating mouse embryo. The cells migrated in the embryo along normal migration routes and contributed to functional pigment cells in the postnatal animal, as demonstrated by coat color contribution. This experimental system represents a novel paradigm that allows studying the developmental potential of human cells under in vivo conditions. Importantly, this platform will allow for the investigation of human diseases in the animal by using patient-derived iPSCs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 25, 2016
- Source ID
- 10.1073/pnas.1525518113
Entities
People
- Dongdong Fu
- Johanna Goldmann
- Katherine J. Wert
- Malkiel A. Cohen
- Rudolf Jaenisch
- Styliani Markoulaki
- Yosef Buganim
Organizations
- Congressionally Directed Medical Research Programs
- Massachusetts Institute of Technology
- National Cancer Institute
- National Institutes of Health
- Simons Foundation
- Whitehead Institute