Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation
Abstract
DNA epigenetic modifications in the forms of 5-methylcytosine (5mC) and 5-hydroxylmethylcytosine (5hmC) play crucial regulatory functions in the mammalian genome. Here we developed an ultrasensitive single-molecule epigenetic imaging technology for detecting and quantifying 5hmC and 5mC. By conducting single-molecule fluorescence resonance energy transfer experiments, we discovered high levels of adjacent and opposing methylated and hydroxymethylated CpG sites in the mouse genome, a previously unappreciated structure which may play an important role in gene regulation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 28, 2016
- Source ID
- 10.1073/pnas.1600223113
Entities
People
- Axel T. Brunger
- Chun-xiao Song
- Jiajie Diao
- Stephen Quake
Organizations
- National Institutes of Health
- Stanford University
- United States Department of Defense