Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli
Abstract
This work presents the genome-wide replacement of all rare AGR (AGA and AGG) arginine codons in the essential genes of Escherichia coli with synonymous CGN alternatives. Synonymous codon substitutions can lethally impact noncoding function by disrupting mRNA secondary structure and ribosomal binding site-like motifs. Here we quantitatively define the range of tolerable deviation in these metrics and use this relationship to provide critical insight into codon choice in recoded genomes. This work demonstrates that genome-wide removal of AGR is likely to be possible and provides a framework for designing genomes with radically altered genetic codes.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 06, 2016
- Source ID
- 10.1073/pnas.1605856113
Entities
People
- Christopher J. Gregg
- Daniel B. Goodman
- Dieter Söll
- Farren J Isaacs
- George M. Church
- Gleb Kuznetsov
- Joshua A. Mosberg
- Lakshmi Narasimhan Govindarajan
- Marc J. Lajoie
- Matthieu Landon
- Michael G. Napolitano
- Oscar Vargas-rodriguez
Organizations
- Arnold and Mabel Beckman Foundation
- DuPont
- Harvard Medical School
- Harvard University
- Lynch Foundation
- Mines ParisTech
- National Institute of General Medical Sciences
- National Science Foundation
- National University of Singapore
- United States Department of Defense
- United States Department of Energy
- Yale University