Chromosomal landscape of UV damage formation and repair at single-nucleotide resolution
Abstract
UV-induced DNA lesions are an important contributor to melanomas and other skin cancers. To understand how UV damage leads to cancer-associated mutations, it is important to know how the chromosomal landscape influences initial UV damage formation and repair. We have developed a UV damage mapping procedure to precisely map UV damage throughout the genome. We used this method to map the genome-wide distribution of UV lesions in yeast, a model eukaryote. We found that UV damage is not uniformly distributed, but that damage formation is significantly modulated in a predictable way by nucleosomes and DNA-bound transcription factors. Additionally, genome-wide analysis of removal of UV lesions indicates that repair is significantly inhibited near the center of strongly positioned nucleosomes.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 25, 2016
- Source ID
- 10.1073/pnas.1606667113
Entities
People
- John J Wyrick
- Michael J. Smerdon
- Peng Mao
- Steven A Roberts
Organizations
- National Institute of Environmental Health Sciences
- United States Department of Defense
- Washington State University