Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR
Abstract
The essence of the adaptive immune response depends on the specificity of antigen receptors. This report identifies shared alpha–beta T-cell receptor (TCR) pairs from the tissues of HLA-A2 + patients with breast cancer and control donors. Using an emulsion-based RT-PCR assay, we analyzed TCR sequences from tissues ex vivo. We identified multiple TCR pairs shared between tumors, but not control samples. Although recent reports have concluded that anticancer T-cell responses depend on patient-specific mutation-associated neoantigens, this study provides evidence that T cells also recognize shared antigens. This approach has broad application to a variety of research questions where the end goal is to examine T-cell repertoires and/or identify T-cell antigens.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 15, 2016
- Source ID
- 10.1073/pnas.1606994113
Entities
People
- Andrew P. Fontenot
- Brandon L. Moore
- Colt A. Egelston
- Daniel J. Munson
- Diana L. Simons
- Dmitri V. Rozanov
- Grecia Jimenez
- Jill E Slansky
- John H. Yim
- John W. Kappler
- Kami E. Chiotti
- Michael T. Falta
- Paul R. Reynolds
- Paul Spellman
- Peter P Lee
- Sonia M. Leach
- Taizo A. Nakano
- Tullia C. Bruno
- Virginia F Borges
- Zuly E. Parra
Organizations
- Congressionally Directed Medical Research Programs
- Department of Surgery
- National Jewish Health
- Oregon Health & Science University
- University of Colorado
- University of Colorado School of Medicine