Genome-wide CRISPR screen identifies HNRNPL as a prostate cancer dependency regulating RNA splicing

Abstract

Alternative RNA splicing and the spliceosome machinery have been implicated in cancer progression. A genome-wide CRISPR screen identified the RNA processing factor heterogeneous nuclear ribonucleoprotein L (HNRNPL) as required for prostate cancer growth by regulating alternative RNA splicing and circular RNA formation. HNRNPL and its RNA clients are overexpressed during prostate cancer progression, supporting their potential role as therapeutic targets.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jun 13, 2017
Source ID
10.1073/pnas.1617467114

Entities

People

  • Felix Y. Feng
  • Housheng Hansen He
  • Laura Cato
  • Massimo Loda
  • Maura B. Cotter
  • Michaela Bowden
  • Myles A. Brown
  • Peng Zhang
  • Qiu Wu
  • Rosina T Lis
  • Shuang G. Zhao
  • Teng Fei
  • Tengfei Xiao
  • Wei Li
  • Xiaole Shirley Liu
  • Yiwen Chen

Organizations

  • Broad Institute
  • Dana–Farber Cancer Institute
  • Harvard Medical School
  • Harvard T.H. Chan School of Public Health
  • National Cancer Institute
  • Northeastern University
  • Tongji University
  • United States Department of Defense
  • University of Michigan
  • University of Texas at Austin
  • University of Toronto

Tags

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology