Design principles for robust vesiculation in clathrin-mediated endocytosis
Abstract
Plasma membrane tension plays an important role in various biological processes. In particular, recent experimental studies have shown that membrane tension inhibits membrane budding processes like clathrin-mediated endocytosis. We have identified a mathematical relationship between the curvature-generating capability of the protein coat and membrane tension that can predict whether the coat alone is sufficient to produce closed buds. Additionally, we show that a combination of increased coat rigidity and applied force from actin polymerization can produce closed buds at high membrane tensions. These findings are general to any membrane-budding process, suggesting that biology has evolved to take advantage of a set of physical design principles to ensure robust vesicle formation across a range of organisms and mechanical environments.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 26, 2017
- Source ID
- 10.1073/pnas.1617705114
Entities
People
- David G Drubin
- George Oster
- Julian E. Hassinger
- Padmini Rangamani
Organizations
- Air Force Office of Scientific Research
- National Institutes of Health
- National Science Foundation
- University of California, San Diego