Antibacterial photosensitization through activation of coproporphyrinogen oxidase

Abstract

Skin and soft tissue infections (SSTIs) account for a majority of visits to hospitals and clinics in the United States and are typically caused by Gram-positive pathogens. Recently, it was discovered that Gram-positive bacteria use a unique pathway to synthesize the critical cellular cofactor heme. The divergence of the heme biosynthesis pathways between humans and Gram-positive bacteria provides a unique opportunity for the development of new antibiotics targeting this pathway. We report here the identification of a small-molecule activator of coproporphyrinogen oxidase (CgoX) from Gram-positive bacteria that induces accumulation of coproporphyrin III and leads to photosensitization of Gram-positive pathogens. In combination with light, CgoX activation reduces bacterial burden in murine models of SSTI.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jul 24, 2017
Source ID
10.1073/pnas.1700469114

Entities

People

  • Audra R. Fullen
  • Brendan F. Dutter
  • D. Borden Lacy
  • Dennis J. Horvath Jr.
  • E Duco Jansen
  • Eric P. Skaar
  • Gary A. Sulikowski
  • Harry A. Dailey
  • Ivelin S. Georgiev
  • J. Logan Jenkins
  • Jeremy B Ford
  • Jocelyn Simpson
  • Kenneth J. Salleng
  • Lisa J. Lojek
  • Matthew Albertolle
  • Matthew C. Surdel
  • Pedro L. Teixeira
  • Raju Nagarajan

Organizations

  • National Institutes of Health
  • University of Georgia
  • Vanderbilt University
  • Walter Reed Army Institute of Research

Tags

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Oncology