Diagnosis of prostate cancer by desorption electrospray ionization mass spectrometric imaging of small metabolites and lipids
Abstract
Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is a label-free molecular imaging technique that provides a window into the biochemical processes present in benign and malignant prostate tissue. This is important both in improving the understanding of tissue biology and in achieving rapid cancer diagnosis. We applied DESI-MSI to record lipid, carbohydrate, and most importantly, small metabolite images from 54 normal and malignant prostate tissue specimens. Several Krebs cycle intermediates were present at different concentrations in prostate cancer compared with normal tissue. Statistical calculations identified panels of metabolites that could readily distinguish prostate cancer from normal tissue with nearly 90% accuracy in a validation set. The results also indicated that the ratio of glucose to citrate ion signals could be used to accurately identify prostate cancer.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 14, 2017
- Source ID
- 10.1073/pnas.1700677114
Entities
People
- Christian A Kunder
- Geoffrey A. Sonn
- James D Brooks
- Richard Fan
- Richard Zare
- Robert J. Tibshirani
- Rosalie Nolley
- Shibdas Banerjee
Organizations
- Air Force Office of Scientific Research
- National Institutes of Health
- Stanford University