First critical repressive H3K27me3 marks in embryonic stem cells identified using designed protein inhibitor
Abstract
We describe an approach to blocking protein–protein interactions in living cells and use it to probe the earliest stages of epigenetic regulation in stem cell differentiation. We describe a computationally designed protein that tightly binds EED and disrupts PRC2 function in both cancer and stem cells. Expression of the binder at different stem cell stages identifies the first critical repressive H3K27me3 mark in embryonic development.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 01, 2017
- Source ID
- 10.1073/pnas.1706907114
Entities
People
- Aaron M. Robitaille
- Amy Ferreccio
- Chao Xu
- Cheng Dong
- Cristina Valensisi
- Damien Detraux
- David Baker
- Hannele Ruohola-Baker
- James D Moody
- Jinrong Min
- Julie Mathieu
- Lauren Carter
- Licheng Zhu
- Luke T. Dang
- R. David Hawkins
- Randall T. Moon
- Shiri Levy
- Sonia Sidhu
- Stuart H. Orkin
- Wolfram Tempel
- Woo-Jin Kim
- Yalan Xing
- Yuliang Wang
Organizations
- Boston Children's Hospital
- Dana–Farber Cancer Institute
- Harvard Medical School
- Howard Hughes Medical Institute
- Jinggangshan University
- University of Science and Technology of China
- University of Toronto
- University of Washington