Escherichia colicultures maintain stable subpopulation structure during long-term evolution

Abstract

Understanding how microbes adapt to novel environments is essential to understanding acute bacterial infection and long-term disease, as genetic architecture underlying the production and maintenance of genetic variation influences a population’s potential for adaptation. In this in-depth analysis of a highly replicatedEscherichia colilong-term evolution experiment, we observe rapid diversification into stable subpopulations in response to several environmental variables. This niche separation creates novel genetic backgrounds upon which new traits, such as differential nutrient utilization or antimicrobial resistance, can arise. The observed genetic changes, in a simple and tractable experimental system, mimic events known to occur during bacterial infections.

Document Details

Document Type
Pub Defense Publication
Publication Date
Apr 30, 2018
Source ID
10.1073/pnas.1708371115

Entities

People

  • Brian I. Choi
  • Jonathan A. Karty
  • Megan G. Behringer
  • Michael Lynch
  • Samuel F. Miller
  • Thomas G. Doak
  • Wanfeng Guo

Organizations

  • Arizona State University
  • Army Research Office
  • Indiana University
  • National Institute of General Medical Sciences

Tags

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biology
  • Systems Analysis and Design

Technology Areas

  • Biotechnology