Targeting CXCR4-dependent immunosuppressive Ly6C low monocytes improves antiangiogenic therapy in colorectal cancer

Abstract

The survival benefit of antiangiogenic therapies for cancer patients has been limited, potentially due to intrinsic/acquired resistance. Deciphering and targeting resistance mechanisms are critical to improving treatment outcome, especially in cancers where antiangiogenic therapies are standard of care, such as colorectal cancer (CRC). Consistent with our clinical findings, we found up-regulation of CXCL12/CXCR4 in orthotopic CRC models and conditional Apc mutant spontaneous rectal tumors after anti-VEGFR2 treatment. CXCR4 signaling recruited immunosuppressive innate immune cells such as Ly6C low monocytes and Ly6G + neutrophils to the CRCs, conferring resistance to VEGFR2 blockade. Furthermore, we successfully targeted these pathways genetically and pharmacologically, including with an FDA-approved agent Plerixafor (AMD3100), which significantly enhanced treatment response. These strategies have the potential for rapid clinical translation.

Document Details

Document Type
Pub Defense Publication
Publication Date
Sep 12, 2017
Source ID
10.1073/pnas.1710754114

Entities

People

  • Andrew D. Luster
  • Christopher G. Willett
  • Dai Fukumura
  • Elizabeth Y. Beech
  • Euiheon Chung
  • Jeffrey W. Clark
  • Joao Incio
  • Jun Ki Kim
  • Keehoon Jung
  • Kosuke Kawaguchi
  • Matthias Pinter
  • Nuh N. Rahbari
  • Rakesh Jain
  • Seok‐Hyun Yun
  • Takahiro Heishi
  • Timothy P Padera
  • William W Ho
  • Yuhui Huang

Organizations

  • Duke University
  • Harvard Medical School
  • Harvard University
  • Massachusetts Institute of Technology
  • National Cancer Institute
  • National Institutes of Health
  • United States Army

Tags

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology and Pathology
  • Oncology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech