Across the tree of life, radiation resistance is governed by antioxidant Mn 2+ , gauged by paramagnetic resonance
Abstract
Decades of functional genomic efforts have failed to predict the ability of cells to survive ionizing radiation (IR). Evidence is mounting that small high-symmetry antioxidant complexes of manganous ions with metabolites (H-Mn 2+ ) are responsible for cellular IR resistance, and that H-Mn 2+ protects the proteome, not the genome, from IR-induced reactive oxygen species. We show that the amount of H-Mn 2+ in nonirradiated living cells is readily gauged by electron paramagnetic resonance (EPR) spectroscopy and highly diagnostic of their DNA repair efficiency and survival after gamma-radiation exposure. This spectroscopic measure of cellular H-Mn 2+ content is the strongest known biological indicator of cellular IR resistance between and within organisms across the three domains of the tree of life, with potential applications including optimization of radiotherapy.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 17, 2017
- Source ID
- 10.1073/pnas.1713608114
Entities
People
- Ajay Sharma
- Andrew Ozarowski
- Brian M. Hoffman
- Cene Gostinčar
- Elena K. Gaidamakova
- Igor Shuryak
- Isabel H. Conze
- Jocelyne DiRuggiero
- Michael J. Daly
- Nina Gunde-cimerman
- Olga Grichenko
- Polina Klimenkova
- Robert P. Volpe
- Rok Tkavc
- Vera Y. Matrosova
- Veronika Hoeke
Organizations
- Air Force Office of Scientific Research
- Bielefeld University
- Columbia University
- Defense Threat Reduction Agency
- Florida State University
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Johns Hopkins University
- National Institutes of Health
- National Science Foundation
- Northwestern University
- Uniformed Services University of the Health Sciences
- University of Ljubljana