Layered dynamic regulation for improving metabolic pathway productivity in Escherichia coli
Abstract
Optimization of the engineered pathway for production of D-glucaric acid, a precursor to nylons and detergents, requires strategies that manage pathway competition with glycolysis as well as the stability and activity of the rate-controlling enzyme. To address both of these challenges, two orthogonal, autonomous, and tunable dynamic regulation strategies were layered to produce the highest reported glucaric acid titers in Escherichia coli K-12 strains. The implementation of two regulatory circuits harnesses synthetic biology tools to program and optimize cellular behavior and demonstrates the power of multiplexed dynamic control for strain optimization.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 05, 2018
- Source ID
- 10.1073/pnas.1716920115
Entities
People
- Apoorv Gupta
- Kristala L. J. Prather
- Stephanie J Doong
Organizations
- Army Research Office
- Massachusetts Institute of Technology
- National Institutes of Health
- National Science Foundation