Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green
Abstract
Imaging in the shortwave IR (SWIR) spectral window allows the observation of processes deep within living animals. Recent studies have shown that SWIR imaging enables unprecedented imaging opportunities, including contact-free monitoring of vital signs, generation of microvasculature blood flow maps, real-time metabolic imaging, and molecularly targeted imaging. Yet, whereas bright SWIR fluorophores have been developed for preclinical research settings, applications in the clinic have been held back by the conventional belief that no clinically approved fluorophore is available. Here, we show that indocyanine green, a clinically approved near-IR dye, exhibits a remarkable amount of SWIR emission, which enables state-of-the-art SWIR imaging with direct translation potential into clinical settings, and even outperforms other commercially available SWIR emitters.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 06, 2018
- Source ID
- 10.1073/pnas.1718917115
Entities
People
- Collin F Perkinson
- Dai Fukumura
- Daniel Franke
- Jessica A Carr
- Justin R Caram
- Mari Saif
- Meenal Datta
- Moungi Bawendi
- Oliver T. Bruns
- Rakesh Jain
- Vasileios Askoxylakis
Organizations
- Air Force Office of Scientific Research
- Boehringer Ingelheim Foundation
- European Molecular Biology Organization
- Harvard Medical School
- Massachusetts Institute of Technology
- National Heart, Lung, and Blood Institute
- National Institutes of Health
- National Science Foundation
- Office of Science
- Tufts University
- United States Department of Defense