Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids
Abstract
Light–oxygen–voltage (LOV) domain photoreceptors are found ubiquitously in nature and possess highly diverse signaling roles and mechanisms. Here, we show that a class of fungal LOV proteins dynamically associates with anionic plasma membrane phospholipids by a blue light-switched electrostatic interaction. This reversible association is rapidly triggered by blue light and ceases within seconds when illumination ceases. Within the native host, we predict that these proteins regulate G-protein signaling by the controlled recruitment of fused regulator of G-protein signaling (RGS) domains; in applied contexts, we anticipate that engineered chimeric versions of such proteins will be useful for rapid optogenetic membrane localization of fused proteins through direct interaction with the membrane itself, without requiring additional components to direct subcellular localization.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 31, 2018
- Source ID
- 10.1073/pnas.1802832115
Entities
People
- Benjamin S Schuster
- Brian Y. Chow
- Erin E. Berlew
- Kevin H. Gardner
- Spencer T. Glantz
- Zaynab Jaber
Organizations
- City College of New York
- City University of New York
- National Institute of General Medical Sciences
- National Institute of Neurological Disorders and Stroke
- National Institute on Drug Abuse
- National Institutes of Health
- National Science Foundation
- University of Pennsylvania
- W. W. Smith Charitable Trust