Human induced pluripotent stem cell-derived MGE cell grafting after status epilepticus attenuates chronic epilepsy and comorbidities via synaptic integration

Abstract

This study provides evidence that human induced pluripotent stem cell (hiPSC)-derived medial ganglionic eminence (MGE) cell grafting into the hippocampus after status epilepticus can greatly reduce the frequency of spontaneous seizures in the chronic phase through both antiepileptogenic and antiepileptic effects. The antiepileptogenic changes comprised reductions in host interneuron loss, abnormal neurogenesis, and aberrant mossy fiber sprouting, whereas the antiepileptic effects were evident from an increased occurrence of seizures after silencing of graft-derived interneurons. Additional curative impacts of grafting comprised improved cognitive and mood function. The results support the application of autologous human MGE cell therapy for temporal lobe epilepsy. Autologous cell therapy is advantageous as such a paradigm can avoid immune suppression and promote enduring graft–host integration.

Document Details

Document Type
Pub Defense Publication
Publication Date
Dec 17, 2018
Source ID
10.1073/pnas.1814185115

Entities

People

  • Adrian Bates
  • Ashok K. Shetty
  • Bharathi Hattiangady
  • Bing Shuai
  • Darwin J. Prockop
  • Dinesh Upadhya
  • Maheedhar Kodali
  • Olagide W. Castro
  • Sahithi Attaluri
  • Su-chun Zhang
  • Yi Dong

Organizations

  • National Institutes of Health
  • Texas Emerging Technology Fund
  • United States Department of Defense
  • United States Department of Veterans Affairs
  • University of Wisconsin–Madison

Tags

Fields of Study

  • Medicine

Readers

  • Molecular and Cellular Biology
  • Neuroscience

Technology Areas

  • Biotechnology