MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo
Abstract
Mitochondria are intracellular hubs of metabolism that play critical roles in mammals, but knowledge of the metabolic landscape within mitochondria in mammalian tissues remains incomplete. Prior studies have often relied on interrogating metabolites within whole tissue, but this approach does not reveal the behavior of metabolites within specific organelles and cell types. To address this, we generated a transgenic mouse that can express a mitochondrially localized epitope tag (MITO-Tag) with spatiotemporal control to allow for rapid, cell-type-specific immunoisolation of mitochondria from tissues. We demonstrate that these MITO-Tag Mice can be utilized for profiling a variety of molecular components of mitochondria, such as proteins, lipids, and polar metabolites, and thus believe that MITO-Tag Mice will be useful for studying mitochondrial physiology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 12, 2018
- Source ID
- 10.1073/pnas.1816656115
Entities
People
- Andrew L. Cangelosi
- Caroline A Lewis
- Ceren Özerdem
- David M. Sabatini
- Erol C. Bayraktar
- Kıvanç Birsoy
- Lou Baudrier
- Monther Abu-Remaileh
- Sze Ham Chan
- Tenzin Kunchok
- Walter W Chen
Organizations
- American Association for Cancer Research
- American Cancer Society
- Boston Children's Hospital
- Howard Hughes Medical Institute
- Kinship Conservation Fellows
- Koch Institute for Integrative Cancer Research at MIT
- March of Dimes
- Massachusetts Institute of Technology
- National Institutes of Health
- Sidney Kimmel Foundation
- The Pew Charitable Trusts
- The Rockefeller University
- United States Department of Defense
- Whitehead Institute