Tracking the evolution of CNS remyelinating lesion in mice with neutral red dye
Abstract
To develop therapeutic strategies that enhance repair processes during central nervous system (CNS) injury, the molecular and cellular changes occurring at the injury site must be determined. However, precisely isolating the injury site, such as lesions in animal models of demyelination, has been difficult. We developed a simple and powerful method to track and analyze changes that occur within the lesion microenvironment over time. We demonstrated that neutral red-labeled lesions could be selectively isolated and processed for detailed analyses of transcripts, proteins, cell populations, and metabolites. The results of our study can be used to identify molecular pathways that promote CNS repair and the development of therapies to modulate neuroinflammation or enhance regeneration in demyelinating disorders such as multiple sclerosis.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 24, 2019
- Source ID
- 10.1073/pnas.1819343116
Entities
People
- David S. Cha
- Evan M. Miller
- Jeffrey K Huang
- Jingwen Hu
- Katherine A. Kelly
- Maryna Baydyuk
- Reiji Yamazaki
- Victoria N. Smith
Organizations
- Congressionally Directed Medical Research Programs
- Georgetown University
- National Institutes of Health
- National Multiple Sclerosis Society