Mechanical stress compromises multicomponent efflux complexes in bacteria

Abstract

The field of mechanobiology examines how physical forces modulate cell physiology and has traditionally focused on eukaryotic organisms. Here we show that in bacteria, mechanical stresses can interrupt the structure and function of a molecular assembly used by Gram-negative bacteria to survive and grow in the presence of toxins. This work provides evidence that bacteria, like mammalian cells, can respond to mechanical forces through molecular complexes at the cell surface in ways that are relevant to growth. Our observations further suggest that mechanical forces may be used synergistically with other antimicrobials.

Document Details

Document Type
Pub Defense Publication
Publication Date
Nov 26, 2019
Source ID
10.1073/pnas.1909562116

Entities

People

  • Abhishek Srivastava
  • Ace George Santiago
  • Bing Fu
  • Christine E. Harper
  • Christopher Hernandez
  • Chung-yuen Hui
  • Lauren A Genova
  • Lucy M. Wang
  • Melanie F. Roberts
  • Peng Chen
  • Won Jung
  • Xianwen Mao
  • Xuanhao Sun
  • Yu-chern Wong
  • Łukasz Krzemiński

Organizations

  • Army Research Office
  • Cornell University
  • National Institute of Allergy and Infectious Diseases
  • National Institute of General Medical Sciences
  • National Science Foundation
  • Office of the Director

Tags

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Materials Science and Engineering.
  • Microbial Pathology