An integrative approach to the facile functional classification of dorsal root ganglion neuronal subclasses
Abstract
We have used the cellular response to a conopeptide, a highly selective K-channel blocker κM-RIIIJ, as the initial step in an integrated molecular-, cellular-, and systems-level functional analysis to describe and characterize six discrete subclasses of large-diameter mouse dorsal-root ganglion neurons. Analysis of two of these classes (proprioceptors and Aδ–low-threshold mechanoreceptors) by current-clamp electrophysiology and single-cell transcriptome analysis revealed functional differences between the subclasses based on specific molecular components. This integrated strategy is a potentially powerful tool for investigating individual neuronal cell types in functional context. This study also establishes a general approach for defining which specific ion-channel heteromeric combinations are present in different individual neuronal subtypes.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 20, 2020
- Source ID
- 10.1073/pnas.1911382117
Entities
People
- Baldomero Olivera
- Heinrich Terlau
- Kevin Chase
- Lee S. Leavitt
- Mario J. Giacobassi
- Rishi K Alluri
- Rocio K Finol-Urdaneta
- Russell W. Teichert
- Shrinivasan Raghuraman
Organizations
- Kiel University
- National Institute of General Medical Sciences
- United States Department of Defense
- University of Utah
- University of Wollongong