Method to extract multiple states in F 1 -ATPase rotation experiments from jump distributions
Abstract
In single-molecule imaging of the F 1 - ATPase rotation by nanoprobes, there are jumps in the angular position. Their distribution is used in a method for detecting hidden states in the transitions during rotation steps. Complementing stalling experiments, this method reveals, now in free rotation without magnetic tweezers, that the motor can be in either of 2 states at a rotation angle. A comparison between the experimental trajectories and a multistate theory reveals that an 80° substep of the coupled ATP binding and ADP release involves an intermediate state reminiscent of a 3-occupancy structure. Its lifetime ( ∼ 10 μs) is comparable with the frame time of the imaging, so, by detecting this short-lived state, the method provides an increased effective time resolution.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 27, 2019
- Source ID
- 10.1073/pnas.1915314116
Entities
People
- Haibin Su
- Kaicheng Zhu
- Luan Q. Le
- Rudolph A. Marcus
- Sándor Volkán-kacsó
Organizations
- Army Research Office
- Azusa Pacific University
- California Institute of Technology
- Hong Kong University of Science and Technology
- Nanyang Technological University
- Office of Naval Research