Spatiotemporal mapping of bacterial membrane potential responses to extracellular electron transfer
Abstract
Past studies on extracellular electron transfer (EET) have mainly employed bulk techniques, overlooking the subpopulation variation in EET and the potentially complex spatial patterns of activity in microbial communities. Here, by performing simultaneous electrochemical and in vivo single-cell-level fluorescence microscopy, we demonstrate the link between EET and the cell membrane potential, revealing the utility of membrane potential as a single-cell-level bioenergetic indicator of EET by Shewanella oneidensis MR-1 cells. Our findings pave the way for understanding the factors underlying EET at a fundamental level, which could, in turn, lead to improvements in the design of bioelectrochemical systems that enhance their overall performance.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 03, 2020
- Source ID
- 10.1073/pnas.2000802117
Entities
People
- Marko S. Chavez
- Mohamed Y El-Naggar
- Sahand Pirbadian
Organizations
- Air Force Office of Scientific Research
- Office of Naval Research
- University of Southern California