Genetic removal of p70 S6K1 corrects coding sequence length-dependent alterations in mRNA translation in fragile X syndrome mice

Abstract

Fragile X syndrome (FXS) is the most prevalent inherited form of intellectual disability. FXS is caused by loss of fragile X mental retardation protein (FMRP), an RNA-binding protein that represses translation. Correspondingly, genetic removal of the translation-stimulating p70 S6 kinase 1 (S6K1) in FXS mice restores translational homeostasis and rescues synaptic and behavioral phenotypes. However, the mechanisms of FMRP function are in dispute, and the molecular basis of the rescue in protein synthesis with reduction of S6K1 is unknown. Here, by carrying out ribosome profiling and molecular assays, we reveal the molecular mechanisms by which reduction of S6K1 corrects altered protein synthesis in FXS. Our study elucidates the molecular foundations of a model for correction of pathophysiological features of FXS.

Document Details

Document Type
Pub Defense Publication
Publication Date
Apr 27, 2021
Source ID
10.1073/pnas.2001681118

Entities

People

  • Eric Klann
  • Francesco Longo
  • Sameer Aryal

Organizations

  • National Institutes of Health
  • New York University
  • United States Department of Defense

Tags

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Theoretical Analysis.
  • Traumatic Brain Injury (TBI) and Cognitive Aging in the Guam and Border Populations Affected by Alzheimer's Disease and Tau-Associated Dementias.

Technology Areas

  • Biotechnology