Fluorescent Janus emulsions for biosensing of Listeria monocytogenes
Abstract
Listeria has been a strong foodborne pathogen for years and can lead to severe diseases. Efficient sensing methods for quick detections of Listeria are still in demand. Here we designed a biosensor that installed Listeria antibodies on the surface of hydrocarbon phase of Janus droplets. We also dissolved a pair of blocking and emissive dyes in the hydrocarbon and fluorocarbon phases of Janus droplets. With the existence of Listeria , Janus droplets will form agglutinations and tilt that could be easily tracked by fluorimeter. Our work provides a sensitive, simple, and quick method for the detection of Listeria .
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 15, 2020
- Source ID
- 10.1073/pnas.2002623117
Entities
People
- Jie Li
- Kosuke Yoshinaga
- Suchol Savagatrup
- Timothy M. Swager
- Zachary Nelson
Organizations
- Massachusetts Institute of Technology
- National Science Foundation
- Office of Naval Research Global