High-throughput photocapture approach for reaction discovery
Abstract
Mass spectrometry (MS) has inspired powerful modes of reaction discovery. Chemists often rely on MS methods, such as liquid-chromatography MS and gas chromatography MS, to rapidly assess hundreds of small-scale reactions. These chromatography-based MS techniques are preferred as they typically provide data consistent with scaled-up yields. Other MS approaches that more rapidly analyze reactions frequently provide results inconsistent with scaled-up yields. Herein we describe the development of a high-throughput platform that overcomes the analytical bottleneck presented by chromatography-based MS techniques while retaining consistency with scaled-up results. Our MS method, which combines diazirine photocapture and self-assembled monolayers for desorption/ionization MS, enabled the analysis of ∼2,000 reactions in <2.5 h to realize an intermolecular photoredox reaction.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 01, 2020
- Source ID
- 10.1073/pnas.2003347117
Entities
People
- Alison A Bayly
- Benjamin R. Mcdonald
- Karl A Scheidt
- Milan Mrksich
Organizations
- Defense Advanced Research Projects Agency
- National Institute of General Medical Sciences
- Northwestern University