Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach
Abstract
Traditional genetic modulation approaches are typically restricted to binary perturbations, single-sided titrations (either graded up- or down-regulation), or individual gene expression modulation. Here, we utilize a library approach that is able to simultaneously modulate gene expression in a metabolism-wide manner. This library, when coupled with next-generation sequencing, allows for the identification of gene perturbations that would have been missed by classic approaches. For the examples tested, this library identified targets that improved growth on alternative carbon sources as well as improved production of betaxanthins.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 20, 2020
- Source ID
- 10.1073/pnas.2007413117
Entities
People
- Emily K Bowman
- Ernst Oberortner
- Hal S. Alper
- Jan-fang Cheng
- Matthew Deaner
- Robert Evans
- Yasuo Yoshikuni
Organizations
- Air Force Office of Scientific Research
- Joint Genome Institute
- United States Department of Energy
- University of Texas at Austin