Laser spectroscopic technique for direct identification of a single virus I: FASTER CARS
Abstract
Surface features of a virus are very important in determining its virility. For example, the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds to the ACE2 receptor site of the host cell with a much stronger affinity than did the original SARS virus. Thus, it is clearly important to understand the virion surface structure. To that end, the present paper combines the spatial resolution of atomic force microscopy and the spectral resolution of coherent Raman spectroscopy. This combination of tip-enhanced microscopy using femtosecond adaptive spectroscopic techniques for coherent anti-Stokes Raman scattering (FAST CARS) with enhanced resolution (FASTER CARS) allows us to map a single virus particle with nanometer resolution and chemical specificity.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 22, 2020
- Source ID
- 10.1073/pnas.2013169117
Entities
People
- Alexei Sokolov
- Dana Cialla-may
- Jürgen Popp
- Marlan Scully
- Roland Zell
- Stefanie Deinhardt-Emmer
- Tanja Deckert-gaudig
- Volker Deckert
- Zhenhuan Yi
Organizations
- Air Force Office of Scientific Research
- Baylor University
- Friedrich Schiller University Jena
- German Research Foundation
- Jena University Hospital
- King Abdulaziz City for Science and Technology
- Leibniz Institute of Photonic Technology
- National Science Foundation
- Office of Naval Research
- Robert A. Welch Foundation