Rapid isothermal amplification and portable detection system for SARS-CoV-2
Abstract
An important limitation of current assays for the detection of SARS-CoV-2 stems from their reliance on time-consuming, labor-intensive, and laboratory-based protocols for viral isolation, lysis, and removal of inhibiting materials. While RT-PCR remains the gold standard for performing clinical diagnostics to amplify the RNA sequences, there is an urgent need for alternative testing platforms that are rapid, accurate, simple, and portable. Here, we demonstrate isothermal RT-LAMP nucleic acid-based detection of SARS-CoV-2 with an additively manufactured cartridge and a smartphone-based instrument for testing that can be performed at the point of sample collection.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 31, 2020
- Source ID
- 10.1073/pnas.2014739117
Entities
People
- Anurup Ganguli
- Ariana Mostafa
- Brian T. Cunningham
- Enrique Valera
- Fu Sun
- Jacob Berger
- Mehmet Yalçın Aydın
- Nick Holonyak Jr.
- Rashid Bashir
- Sarah A. Stewart De Ramirez
- William P King
Organizations
- Carle-Illinois College of Medicine
- National Institute of Allergy and Infectious Diseases
- National Science Foundation
- United States Department of Agriculture
- University of Illinois Urbana–Champaign