Allosteric cooperation in a de novo-designed two-domain protein
Abstract
A major mechanism of evolution involves fusing genes that encode single-domain proteins to create multidomain structures that achieve new functions. Here, we develop methods to design multidomain proteins entirely from scratch and achieve the premier de novo design of an allosterically regulated phenol oxidase that responds to the binding of a synthetic porphyrin.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 14, 2020
- Source ID
- 10.1073/pnas.2017062117
Entities
People
- Angela Lombardi
- Fabio Pirro
- James Lincoff
- Lijun Liu
- Marco Chino
- Michael Grabe
- Michael Therien
- Nathan Schmidt
- Nicholas F Polizzi
- William DeGrado
- Zachary X Widel
Organizations
- Air Force Office of Scientific Research
- Duke University
- National Institutes of Health
- National Science Foundation
- Peking University
- University of California
- University of Naples Federico II