Prdm16 is a critical regulator of adult long-term hematopoietic stem cell quiescence

Abstract

Regulation of quiescence is critical for the maintenance of adult HSCs, and the underlying mechanisms are poorly understood. Using a novel mouse conditional knockout allele of transcription factor gene Prdm16 , we show that its deletion in the adult hematopoietic system led to a gradual loss of adult HSCs over time. This loss of adult HSCs was associated with their significantly increased cycling. We further found that Prdm16 promotes the quiescence of adult HSCs by activating the transcription of HSC cell cycle inhibitors including Cdkn1a and Egr1 . Our study identifies Prdm16 as a critical regulator of adult HSC quiescence.

Document Details

Document Type
Pub Defense Publication
Publication Date
Dec 02, 2020
Source ID
10.1073/pnas.2017626117

Entities

People

  • Bjorg Gudmundsdottir
  • Kevin Oakley
  • Kristbjorn O Gudmundsson
  • Lino Tessarollo
  • Nancy A. Jenkins
  • Neal G. Copeland
  • Nhu Nguyen
  • Pentao Liu
  • Yang Du
  • Yufen Han

Organizations

  • National Cancer Institute
  • National Heart, Lung, and Blood Institute
  • Uniformed Services University of the Health Sciences
  • University of Hong Kong
  • University of Texas at Austin

Tags

Readers

  • Medical or Health Care Field.
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology