Thymine dissociation and dimer formation: A Raman and synchronous fluorescence spectroscopic study
Abstract
Bacteria inactivation by ultraviolet light is caused due to the formation of thymine dimers that inhibit DNA replication. We have used spectroscopic means for the identification of thymine dimer(s) that may pave the way for fast and definite identification of the ratio of live and dead bacteria, as suggested by our previous spectroscopic studies. In the present study, the dissociation of thymine to thymine dimers and (6-4) photoproducts was detected by means of a Raman, absorption, and synchronous fluorescence study. These data provide a fast method for determining, in situ, the reaction mechanism and final photoproducts formed as a function of UV irradiation dose.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 01, 2021
- Source ID
- 10.1073/pnas.2025263118
Entities
People
- Anushka Nagpal
- Dinesh Dhankhar
- Jie Chen
- Peter M. Rentzepis
- Run-Ze Li
- Thomas C. Cesario
Organizations
- Air Force Office of Scientific Research
- Robert A. Welch Foundation
- Shanghai Jiao Tong University
- Texas Engineering Experiment Station
- University of California