Sorting for secreted molecule production using a biosensor-in-microdroplet approach
Abstract
The design–build–test cycle of metabolic engineering is swiftly becoming test limited owing to advances in DNA synthesis and library design. At the same time, improving extracellular production requires high-throughput studies to detect extracellular (not intracellular) content. Traditional approaches that utilize chromatography-based analysis or plate-based fluorescence assays are relatively throughput limited and require a high degree of liquid handling. These limitations are avoided here by creating a generalizable approach for small molecule production screening. Namely, this paradigm combines “off-the-shelf” biosensors with large library screening from producing cells in a cell-type agnostic manner.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 02, 2021
- Source ID
- 10.1073/pnas.2106818118
Entities
People
- Claire M Palmer
- Emily K Bowman
- Frank F. Craig
- Hal S. Alper
- James M. Wagner
- Lauren T. Cordova
- Matthew Deaner
- Shuo-Fu Yuan
- Simon d’Oelsnitz
- Xin Li
Organizations
- Air Force Office of Scientific Research
- Lawrence Berkeley National Laboratory
- University of Texas at Austin