Conformation-driven strategy for resilient and functional protein materials

Abstract

We provide insights into the sequence–conformation–property relationship that is central to the mechanical properties of protein elastomers. We find that a high content of glycine residue alone, not including proline, is sufficient for achieving near-perfect resilience. The content of proline residue may be associated with the metastability of random coils. Also, Raman spectroscopy, as a potent tool for investigating the conformation–property relationship, gives rise to a direct correlation between semiquantitative Raman features and the magnitude of elastic resilience. Moreover, metastable conformation or conformational polymorphism is useful to develop continuously and mechanically graded protein materials that may exhibit unique structural merits. This work underlies the exploitation of natural and de novo–designed sequences for protein elastomers and materials.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 24, 2022
Source ID
10.1073/pnas.2115523119

Entities

People

  • David L. Kaplan
  • Jaewon Choi
  • John S.K. Yuen
  • Peggy Cebe
  • Xiaocheng Jiang
  • Xuan Mu
  • Yixin Zhang
  • Yu Shrike Zhang

Organizations

  • Air Force Office of Scientific Research
  • Army Research Office
  • Harvard Medical School
  • Office of Extramural Research
  • Tufts University

Tags

Readers

  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry
  • Systems Analysis and Design