Single molecule tracking of bacterial cell surface cytochromes reveals dynamics that impact long-distance electron transport
Abstract
Multiheme cytochromes inShewanella oneidensisMR-1 transport electrons across the cell wall, in a process called extracellular electron transfer. These electron conduits can also enable electron transport along and between cells. While the underlying mechanism is thought to involve a combination of electron hopping and lateral diffusion of cytochromes along membranes, these diffusive dynamics have never been observed in vivo. Here, we observe the mobility of quantum dot-labeled cytochromes on living cell surfaces and membrane nanowires, quantify their diffusion with single-particle tracking techniques, and simulate the contribution of these dynamics to electron transport. This work reveals the impact of redox molecule dynamics on bacterial electron transport, with implications for understanding and harnessing this process in the environment and bioelectronics.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 03, 2022
- Source ID
- 10.1073/pnas.2119964119
Entities
People
- Fabien Pinaud
- Grace W Chong
- Lori Zacharoff
- Mohamed Y El-Naggar
- Sahand Pirbadian
- Yunke Zhao
Organizations
- University of Southern California