Cancer cell CCR2 orchestrates suppression of the adaptive immune response

Abstract

C-C chemokine receptor type 2 (CCR2) is expressed on monocytes and facilitates their recruitment to tumors. Though breast cancer cells also express CCR2, its functions in these cells are unclear. We found that Ccr2 deletion in cancer cells led to reduced tumor growth and approximately twofold longer survival in an orthotopic, isograft breast cancer mouse model. Deletion of Ccr2 in cancer cells resulted in multiple alterations associated with better immune control: increased infiltration and activation of cytotoxic T lymphocytes (CTLs) and CD103+ cross-presenting dendritic cells (DCs), as well as up-regulation of MHC class I and down-regulation of checkpoint regulator PD-L1 on the cancer cells. Pharmacological or genetic targeting of CCR2 increased cancer cell sensitivity to CTLs and enabled the cancer cells to induce DC maturation toward the CD103+ subtype. Consistently, Ccr2−/− cancer cells did not induce immune suppression in Batf3−/− mice lacking CD103+ DCs. Our results establish that CCR2 signaling in cancer cells can orchestrate suppression of the immune response.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jul 15, 2020
Source ID
10.1084/jem.20181551

Entities

People

  • Ana S Almeida
  • Anaïs Eberhardt
  • Arnaud Pommier
  • Camila O Dos Santos
  • Douglas T Fearon
  • Emilis Bružas
  • John E. Wilkinson
  • Linda Van Aelst
  • Mikala Egeblad
  • Miriam R. Fein
  • Ran Yan
  • Xue-yan He

Organizations

  • Cold Spring Harbor Laboratory
  • Lustgarten Foundation for Pancreatic Cancer Research
  • National Cancer Institute
  • Rita Allen Foundation
  • Simons Foundation
  • Stony Brook University
  • United States Department of Defense
  • University of Cambridge
  • University of Michigan
  • V Foundation for Cancer Research
  • Watson School of Biological Sciences
  • Weill Cornell Medicine
  • École Normale Supérieure de Lyon

Tags

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Life Cycle Cost Analysis
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech